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ficoll–conray density gradient centrifugation  (Conray Inc)

 
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    Conray Inc ficoll–conray density gradient centrifugation
    Ficoll–Conray Density Gradient Centrifugation, supplied by Conray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ficoll-conray+density+gradient+centrifugation/ficoll+conray/pm38202302-103-19-19
    Average 90 stars, based on 1 article reviews
    ficoll–conray density gradient centrifugation - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Isolation:

    Article Title: Comparison of the proliferation, cytotoxic activity and cytokine secretion function of cascade primed immune cells and cytokine-induced killer cells in vitro
    Article Snippet: Ficoll-Conray density gradient centrifugation (50–60 ml peripheral blood, density 1.077±0.001 g/ml) was performed to obtain three separate suspensions of peripheral blood mononuclear cells (PBMCs).

    Article Title: Identification of Programmed Death Ligand 1–derived Peptides Capable of Inducing Cancer-reactive Cytotoxic T Lymphocytes From HLA-A24+ Patients With Renal Cell Carcinoma
    Article Snippet: Molecular therapy targeting tumor angiogenesis has been the standard treatment for metastatic renal cell carcinoma (mRCC).. However, despite their significant antitumor effects, most of patients with mRCC have not been cured.. Under such circumstances, anticancer immunotherapy has been considered a promising treatment modality for mRCC, and cancer-reactive cytotoxic T lymphocytes (CTLs) are the most powerful effectors among several immune cells.

    Article Title: IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides.
    Article Snippet: A total of 15 ml of blood were drawn before each course and then peripheral-blood mononuclear cells (PbMCs) and blood plasma were isolated by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Novel gene therapy viral vector using non-oncogenic lymphotropic herpesvirus.
    Article Snippet: PBMCs and CBMCs were separated by using Ficoll-Conray density gradient centrifugation [19], cultured in RPMI 1640 medium (Invitrogen, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen) and 10 mg/ml IL2 (ProLEUKIN; Chiron Corp, USA), and activated with 5 mg/ml phytohemagglutinin (PHA; J-Oil Mills, Japan) or on plates coated with 1 mg/ml anti-CD3 antibody (orthoclone OKT3; Janssen).

    Article Title: Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients.
    Article Snippet: We isolated the PBMCs from the heparinized blood of Japanese healthy donors by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells
    Article Snippet: After the fourth vaccination, PBMCs were isolated from blood samples by Ficoll–Conray density gradient centrifugation.

    Article Title: Hypoxia-inducing factor (HIF)-1α-derived peptide capable of inducing cancer-reactive cytotoxic T lymphocytes from HLA-A24 + patients with renal cell carcinoma.
    Article Snippet: Abbreviations: HIFs, hypoxia-inducing factors; RCC, Hippel-Lindau; CTLs, cytotoxic T lymphocytes; mRCC, me interleukin; IFN, interferon; PD-1, programmed cell deat 4, cytotoxic T-lymphocyte-associated protein-4; CA, carbo eral blood mononuclear cells; HDs, healthy donors; H virus; EBV, EPSTEIN-Barr virus; DFO, desferrioxamine.. ⁎ Corresponding authors at: Department of Urolog Medicine, 377-2 Ohno-higashi, Osaka-Sayama, 589-8511 E-mail addresses: minami@med.kindai.ac.jp (T. Minam (H. Uemura).

    Article Title: Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR-511-3p in non-small cell lung cancer.
    Article Snippet: Funding information Key Guiding Project of Hunan Provincial Health Commission, Grant/Award Number: 20201903; Hunan Provincial Natural Science Foundation, Grant/Award Number: 2019JJ40318 Abstract Background: Accumulating evidence suggests that circular RNAs (circRNAs) play important regulatory roles in non-small cell lung cancer (NSCLC).. At present, we aimed to explore the regulatory role of has_circ_0003528 (circ_0003528) in NSCLC.. Methods: Alterations of circ_0003528 expression in NSCLC samples and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

    Isolation:

    Article Title: Comparison of the proliferation, cytotoxic activity and cytokine secretion function of cascade primed immune cells and cytokine-induced killer cells in vitro
    Article Snippet: Ficoll-Conray density gradient centrifugation (50–60 ml peripheral blood, density 1.077±0.001 g/ml) was performed to obtain three separate suspensions of peripheral blood mononuclear cells (PBMCs).

    Article Title: Identification of Programmed Death Ligand 1–derived Peptides Capable of Inducing Cancer-reactive Cytotoxic T Lymphocytes From HLA-A24+ Patients With Renal Cell Carcinoma
    Article Snippet: Molecular therapy targeting tumor angiogenesis has been the standard treatment for metastatic renal cell carcinoma (mRCC).. However, despite their significant antitumor effects, most of patients with mRCC have not been cured.. Under such circumstances, anticancer immunotherapy has been considered a promising treatment modality for mRCC, and cancer-reactive cytotoxic T lymphocytes (CTLs) are the most powerful effectors among several immune cells.

    Article Title: IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides.
    Article Snippet: A total of 15 ml of blood were drawn before each course and then peripheral-blood mononuclear cells (PbMCs) and blood plasma were isolated by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Novel gene therapy viral vector using non-oncogenic lymphotropic herpesvirus.
    Article Snippet: PBMCs and CBMCs were separated by using Ficoll-Conray density gradient centrifugation [19], cultured in RPMI 1640 medium (Invitrogen, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen) and 10 mg/ml IL2 (ProLEUKIN; Chiron Corp, USA), and activated with 5 mg/ml phytohemagglutinin (PHA; J-Oil Mills, Japan) or on plates coated with 1 mg/ml anti-CD3 antibody (orthoclone OKT3; Janssen).

    Article Title: Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients.
    Article Snippet: We isolated the PBMCs from the heparinized blood of Japanese healthy donors by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells
    Article Snippet: After the fourth vaccination, PBMCs were isolated from blood samples by Ficoll–Conray density gradient centrifugation.

    Article Title: Hypoxia-inducing factor (HIF)-1α-derived peptide capable of inducing cancer-reactive cytotoxic T lymphocytes from HLA-A24 + patients with renal cell carcinoma.
    Article Snippet: Abbreviations: HIFs, hypoxia-inducing factors; RCC, Hippel-Lindau; CTLs, cytotoxic T lymphocytes; mRCC, me interleukin; IFN, interferon; PD-1, programmed cell deat 4, cytotoxic T-lymphocyte-associated protein-4; CA, carbo eral blood mononuclear cells; HDs, healthy donors; H virus; EBV, EPSTEIN-Barr virus; DFO, desferrioxamine.. ⁎ Corresponding authors at: Department of Urolog Medicine, 377-2 Ohno-higashi, Osaka-Sayama, 589-8511 E-mail addresses: minami@med.kindai.ac.jp (T. Minam (H. Uemura).

    Article Title: Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR-511-3p in non-small cell lung cancer.
    Article Snippet: Funding information Key Guiding Project of Hunan Provincial Health Commission, Grant/Award Number: 20201903; Hunan Provincial Natural Science Foundation, Grant/Award Number: 2019JJ40318 Abstract Background: Accumulating evidence suggests that circular RNAs (circRNAs) play important regulatory roles in non-small cell lung cancer (NSCLC).. At present, we aimed to explore the regulatory role of has_circ_0003528 (circ_0003528) in NSCLC.. Methods: Alterations of circ_0003528 expression in NSCLC samples and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

    Gradient Centrifugation:

    Article Title: Comparison of the proliferation, cytotoxic activity and cytokine secretion function of cascade primed immune cells and cytokine-induced killer cells in vitro
    Article Snippet: Ficoll-Conray density gradient centrifugation (50–60 ml peripheral blood, density 1.077±0.001 g/ml) was performed to obtain three separate suspensions of peripheral blood mononuclear cells (PBMCs).

    Article Title: Identification of Programmed Death Ligand 1–derived Peptides Capable of Inducing Cancer-reactive Cytotoxic T Lymphocytes From HLA-A24+ Patients With Renal Cell Carcinoma
    Article Snippet: Molecular therapy targeting tumor angiogenesis has been the standard treatment for metastatic renal cell carcinoma (mRCC).. However, despite their significant antitumor effects, most of patients with mRCC have not been cured.. Under such circumstances, anticancer immunotherapy has been considered a promising treatment modality for mRCC, and cancer-reactive cytotoxic T lymphocytes (CTLs) are the most powerful effectors among several immune cells.

    Article Title: IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides.
    Article Snippet: A total of 15 ml of blood were drawn before each course and then peripheral-blood mononuclear cells (PbMCs) and blood plasma were isolated by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Novel gene therapy viral vector using non-oncogenic lymphotropic herpesvirus.
    Article Snippet: PBMCs and CBMCs were separated by using Ficoll-Conray density gradient centrifugation [19], cultured in RPMI 1640 medium (Invitrogen, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen) and 10 mg/ml IL2 (ProLEUKIN; Chiron Corp, USA), and activated with 5 mg/ml phytohemagglutinin (PHA; J-Oil Mills, Japan) or on plates coated with 1 mg/ml anti-CD3 antibody (orthoclone OKT3; Janssen).

    Article Title: Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients.
    Article Snippet: We isolated the PBMCs from the heparinized blood of Japanese healthy donors by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells
    Article Snippet: After the fourth vaccination, PBMCs were isolated from blood samples by Ficoll–Conray density gradient centrifugation.

    Article Title: Hypoxia-inducing factor (HIF)-1α-derived peptide capable of inducing cancer-reactive cytotoxic T lymphocytes from HLA-A24 + patients with renal cell carcinoma.
    Article Snippet: Abbreviations: HIFs, hypoxia-inducing factors; RCC, Hippel-Lindau; CTLs, cytotoxic T lymphocytes; mRCC, me interleukin; IFN, interferon; PD-1, programmed cell deat 4, cytotoxic T-lymphocyte-associated protein-4; CA, carbo eral blood mononuclear cells; HDs, healthy donors; H virus; EBV, EPSTEIN-Barr virus; DFO, desferrioxamine.. ⁎ Corresponding authors at: Department of Urolog Medicine, 377-2 Ohno-higashi, Osaka-Sayama, 589-8511 E-mail addresses: minami@med.kindai.ac.jp (T. Minam (H. Uemura).

    Article Title: Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR-511-3p in non-small cell lung cancer.
    Article Snippet: Funding information Key Guiding Project of Hunan Provincial Health Commission, Grant/Award Number: 20201903; Hunan Provincial Natural Science Foundation, Grant/Award Number: 2019JJ40318 Abstract Background: Accumulating evidence suggests that circular RNAs (circRNAs) play important regulatory roles in non-small cell lung cancer (NSCLC).. At present, we aimed to explore the regulatory role of has_circ_0003528 (circ_0003528) in NSCLC.. Methods: Alterations of circ_0003528 expression in NSCLC samples and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

    Clinical Proteomics:

    Article Title: Comparison of the proliferation, cytotoxic activity and cytokine secretion function of cascade primed immune cells and cytokine-induced killer cells in vitro
    Article Snippet: Ficoll-Conray density gradient centrifugation (50–60 ml peripheral blood, density 1.077±0.001 g/ml) was performed to obtain three separate suspensions of peripheral blood mononuclear cells (PBMCs).

    Article Title: Identification of Programmed Death Ligand 1–derived Peptides Capable of Inducing Cancer-reactive Cytotoxic T Lymphocytes From HLA-A24+ Patients With Renal Cell Carcinoma
    Article Snippet: Molecular therapy targeting tumor angiogenesis has been the standard treatment for metastatic renal cell carcinoma (mRCC).. However, despite their significant antitumor effects, most of patients with mRCC have not been cured.. Under such circumstances, anticancer immunotherapy has been considered a promising treatment modality for mRCC, and cancer-reactive cytotoxic T lymphocytes (CTLs) are the most powerful effectors among several immune cells.

    Article Title: IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides.
    Article Snippet: A total of 15 ml of blood were drawn before each course and then peripheral-blood mononuclear cells (PbMCs) and blood plasma were isolated by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Novel gene therapy viral vector using non-oncogenic lymphotropic herpesvirus.
    Article Snippet: PBMCs and CBMCs were separated by using Ficoll-Conray density gradient centrifugation [19], cultured in RPMI 1640 medium (Invitrogen, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen) and 10 mg/ml IL2 (ProLEUKIN; Chiron Corp, USA), and activated with 5 mg/ml phytohemagglutinin (PHA; J-Oil Mills, Japan) or on plates coated with 1 mg/ml anti-CD3 antibody (orthoclone OKT3; Janssen).

    Article Title: Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients.
    Article Snippet: We isolated the PBMCs from the heparinized blood of Japanese healthy donors by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells
    Article Snippet: After the fourth vaccination, PBMCs were isolated from blood samples by Ficoll–Conray density gradient centrifugation.

    Article Title: Hypoxia-inducing factor (HIF)-1α-derived peptide capable of inducing cancer-reactive cytotoxic T lymphocytes from HLA-A24 + patients with renal cell carcinoma.
    Article Snippet: Abbreviations: HIFs, hypoxia-inducing factors; RCC, Hippel-Lindau; CTLs, cytotoxic T lymphocytes; mRCC, me interleukin; IFN, interferon; PD-1, programmed cell deat 4, cytotoxic T-lymphocyte-associated protein-4; CA, carbo eral blood mononuclear cells; HDs, healthy donors; H virus; EBV, EPSTEIN-Barr virus; DFO, desferrioxamine.. ⁎ Corresponding authors at: Department of Urolog Medicine, 377-2 Ohno-higashi, Osaka-Sayama, 589-8511 E-mail addresses: minami@med.kindai.ac.jp (T. Minam (H. Uemura).

    Article Title: Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR-511-3p in non-small cell lung cancer.
    Article Snippet: Funding information Key Guiding Project of Hunan Provincial Health Commission, Grant/Award Number: 20201903; Hunan Provincial Natural Science Foundation, Grant/Award Number: 2019JJ40318 Abstract Background: Accumulating evidence suggests that circular RNAs (circRNAs) play important regulatory roles in non-small cell lung cancer (NSCLC).. At present, we aimed to explore the regulatory role of has_circ_0003528 (circ_0003528) in NSCLC.. Methods: Alterations of circ_0003528 expression in NSCLC samples and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR).

    Cell Culture:

    Article Title: Comparison of the proliferation, cytotoxic activity and cytokine secretion function of cascade primed immune cells and cytokine-induced killer cells in vitro
    Article Snippet: Ficoll-Conray density gradient centrifugation (50–60 ml peripheral blood, density 1.077±0.001 g/ml) was performed to obtain three separate suspensions of peripheral blood mononuclear cells (PBMCs).

    Article Title: Identification of Programmed Death Ligand 1–derived Peptides Capable of Inducing Cancer-reactive Cytotoxic T Lymphocytes From HLA-A24+ Patients With Renal Cell Carcinoma
    Article Snippet: Molecular therapy targeting tumor angiogenesis has been the standard treatment for metastatic renal cell carcinoma (mRCC).. However, despite their significant antitumor effects, most of patients with mRCC have not been cured.. Under such circumstances, anticancer immunotherapy has been considered a promising treatment modality for mRCC, and cancer-reactive cytotoxic T lymphocytes (CTLs) are the most powerful effectors among several immune cells.

    Article Title: IgG response to MHC class I epitope peptides is a quantitative predictive biomarker in the early course of treatment of colorectal cancer using therapeutic peptides.
    Article Snippet: A total of 15 ml of blood were drawn before each course and then peripheral-blood mononuclear cells (PbMCs) and blood plasma were isolated by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Novel gene therapy viral vector using non-oncogenic lymphotropic herpesvirus.
    Article Snippet: PBMCs and CBMCs were separated by using Ficoll-Conray density gradient centrifugation [19], cultured in RPMI 1640 medium (Invitrogen, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Invitrogen) and 10 mg/ml IL2 (ProLEUKIN; Chiron Corp, USA), and activated with 5 mg/ml phytohemagglutinin (PHA; J-Oil Mills, Japan) or on plates coated with 1 mg/ml anti-CD3 antibody (orthoclone OKT3; Janssen).

    Article Title: Identification of CDCA1-derived long peptides bearing both CD4+ and CD8+ T-cell epitopes: CDCA1-specific CD4+ T-cell immunity in cancer patients.
    Article Snippet: We isolated the PBMCs from the heparinized blood of Japanese healthy donors by means of Ficoll-Conray density gradient centrifugation.

    Article Title: Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells
    Article Snippet: After the fourth vaccination, PBMCs were isolated from blood samples by Ficoll–Conray density gradient centrifugation.

    Article Title: Hypoxia-inducing factor (HIF)-1α-derived peptide capable of inducing cancer-reactive cytotoxic T lymphocytes from HLA-A24 + patients with renal cell carcinoma.
    Article Snippet: Abbreviations: HIFs, hypoxia-inducing factors; RCC, Hippel-Lindau; CTLs, cytotoxic T lymphocytes; mRCC, me interleukin; IFN, interferon; PD-1, programmed cell deat 4, cytotoxic T-lymphocyte-associated protein-4; CA, carbo eral blood mononuclear cells; HDs, healthy donors; H virus; EBV, EPSTEIN-Barr virus; DFO, desferrioxamine.. ⁎ Corresponding authors at: Department of Urolog Medicine, 377-2 Ohno-higashi, Osaka-Sayama, 589-8511 E-mail addresses: minami@med.kindai.ac.jp (T. Minam (H. Uemura).

    Article Title: Hsa_circ_0003528 promotes cell malignant transformation and immune escape via increasing oncogene PDL1 through sponging miR-511-3p in non-small cell lung cancer.
    Article Snippet: Funding information Key Guiding Project of Hunan Provincial Health Commission, Grant/Award Number: 20201903; Hunan Provincial Natural Science Foundation, Grant/Award Number: 2019JJ40318 Abstract Background: Accumulating evidence suggests that circular RNAs (circRNAs) play important regulatory roles in non-small cell lung cancer (NSCLC).. At present, we aimed to explore the regulatory role of has_circ_0003528 (circ_0003528) in NSCLC.. Methods: Alterations of circ_0003528 expression in NSCLC samples and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR).



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